Placenta increta: Postpartum monitoring of plasma cell-free fetal DNA.

is more sensitive but requires two steps [two rounds of amplification (PCR LDR) are needed]. The costs for setup are larger (PCR primers and three labeled oligonucleotides are needed per SNP) with respect to PCR/RFLP and allele-specific PCR but are similar for the TaqMan assay. The main advantage consists in the inherent multiplexing capability that can increase throughput, which decreases cost and time per scored SNP. The capability of performing multiple assays on the same slide further enhances this feature. To improve the molecular characterization of AD, several other polymorphisms potentially involved in the disease could be included within this analytical system.